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SMURF1 (Myc-DDK-tagged)-Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Smurf1 (tGFP-tagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (cDNA clone MGC:28022 IMAGE:3660965)
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
SMURF1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Smurf1 (untagged) - Mouse SMAD specific E3 ubiquitin protein ligase 1 (Smurf1), transcript variant 2, (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
SMURF1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SMURF1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Mus musculus gene Smurf1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Smurf1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
qSTAR qPCR primer pairs against Homo sapiens gene SMURF1
