Products

Rabbit Monoclonal Anti-Mouse IgG2a Kappa antibody, clone RM107

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG2a kappa
Conjugation Unconjugated

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Ascites

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Purified

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgG3 antibody, clone RM119

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgG3
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgG2b (gamma-2b) antibody, clone RM108

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (stronger on non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Mouse IgG2b
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, Purified

Applications Flow Cytometry: 20 µg/ml.
Immunohistochemistry on frozen sections: 20 µg/ml.
ELISA: 5 µg/ml as coating Antibody.
Immunoprecipitation.
Reactivities Human
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, APC

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation APC

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, FITC

Applications FLISA: 1/200-1/400. 
Flow Cytometry: ≤ 1 µg/106 cells.
Identification and enumeration of IgG2a+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/1-1/50.
ELISA: from 1/20 upwards.
Western blot: from 1/40 upwards.
Reactivities Human
Conjugation TRITC

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC

Applications To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC

Human IgA (IgAc, A1, A2, Secretory) mouse monoclonal antibody, clone NI 69 and NI 184+NI 69-11+NI 512+NI 194, Ascites

Applications Can be used to identify the presence of IgA and secretory subclasses in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting. 
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards. 
Western blotting: 1/2000-1/10000.
Reactivities Human
Conjugation Unconjugated

Human IgG, IgA, IgM, IgD, IgE mouse monoclonal antibody, clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307, Ascites

Applications Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards.
Western blotting: 1/2000 Upwards.
Reactivities Human
Conjugation Unconjugated

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine
Conjugation Unconjugated