Products

Human IgE (Fc specific) goat polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000.
Reactivities Human
Conjugation HRP

Duck IgM (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for use in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of Duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/3,000.
Immunohistochemistry and Cytochemistry: 1/50-1/250.
Note: This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Duck
Conjugation HRP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP

Applications ELISA: 1/4,000-1/10,000.
Western blotting.
Dot- and Slot-immunoblotting.
Immunohistochemistry on Frozen Sections: 1/10-1/20.
Reactivities Human
Conjugation HRP

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: from 1/20 upwards.  
ELISA: form 1/50 upwards.  
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation HRP

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Monkey IgG (H&L) Antibody Peroxidase

Applications WB: 1:10,000 - 1:60,000
IHC: User Optimized
ELISA: 1:50,000 - 1:100,000
Conjugation HRP

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections. 
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions 
Histochemical and cytochemical Use: 1/100- 1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/400.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used.
Reactivities Mouse
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate.
Western blot (1/5000).
Immunoelectrophoresis.
Reactivities Chicken
Conjugation HRP

Canine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in dog serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Canine
Conjugation HRP

Canine IgG (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in Dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/10000.
Reactivities Canine
Conjugation HRP

Canine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Conjugation HRP

Canine IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation HRP

Monkey IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
Histochemical and cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Monkey
Conjugation HRP