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Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant B, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Products
Purified recombinant protein of Human receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIPK1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human NLR family, pyrin domain containing 3 (NLRP3), transcript variant 3, full length, with C-terminal HIS tag, expressed in E.Coli, 50 ug
| Tag | C-HIS |
| Expression Host | E. coli |
Recombinant protein of human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human gasdermin D (GSDMD), transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human Sp1 transcription factor (SP1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human forkhead box O4 (FOXO4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human prohibitin 2 (PHB2), transcript variant 1, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human Wilms tumor 1 (WT1), transcript variant A, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human androgen receptor (AR), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human wingless-type MMTV integration site family, member 4 (WNT4), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
Purified recombinant protein of Human SMAD family member 1 (SMAD1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of Human activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
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Target Symbol
- VEGFA (12)
- TNF ALPHA (11)
- IL6 (9)
- BDNF (7)
- EOTAXIN 3 (7)
- FABP4 (7)
- IGF1 (7)
- IL4 (7)
- ALPHA SYNUCLEIN (6)
- BMP2 (6)
- BNP (6)
- FGF1 (6)
- IL1 BETA (6)
- IL33 (6)
- NFYA (6)
- NRG1 (6)
- PTPRN (6)
- TGIF (6)
- TRIM72 (6)
- APOLIPOPROTEIN E (5)
- BIRC5 (5)
- CAMK2B (5)
- FERRITIN HEAVY CHAIN (5)
- FGF2 (5)
- HMG1 (5)
- IL15 (5)
- IL3 (5)
- LIF (5)
- PDGFA (5)
- SDF1 (5)
- TRAIL (5)
- TXN (5)
- BLNK (4)
- BMP4 (4)
- DESMIN (4)
- EGR1 (4)
- EIF4E (4)
- FSTL1 (4)
- GABARAP (4)
- GDNF (4)
- GLUTAMINE SYNTHETASE (4)
- HIP2 (4)
- HIST1H2AK (4)
- IL8 (4)
- INTERFERON ALPHA 2 (4)
- MAP1LC3A (4)
- NEUROTROPHIN 3 (4)
- NHP2 (4)
- PADI4 (4)
- PCNA (4)
- PDGF AA (4)
- PEROXIREDOXIN 1 (4)
- PITX2 (4)
- PTPN11 (4)
- RNASE9 (4)
- SONIC HEDGEHOG (4)
- SUMO 3 (4)
- VILIP1 (4)
- VINCULIN (4)
- ZNF75A (4)
- ADA2A (3)
- AMPHIREGULIN (3)
- ANNEXIN VI (3)
- APE1 (3)
- ASB13 (3)
- ASF1A (3)
- ATP5F1C (3)
- BETA ARRESTIN 1 (3)
- BID (3)
- C-MYC (3)
- C15ORF21 (3)
- CALCITONIN (3)
- CANT1 (3)
- CASPASE 1 (3)
- CASPASE 10 (3)
- CD8A (3)
- CDC42SE2 (3)
- CREM (3)
- CSF2 (3)
- CSNK1G2 (3)
- CSPS (3)
- CXCL14 (3)
- DEOXYRIBONUCLEASE I LIKE 1 (3)
- EGF (3)
- EIF1A (3)
- EIF4EBP1 (3)
- ELF2 (3)
- FAM104B (3)
- FERRITIN LIGHT CHAIN (3)
- FGF21 (3)
- FGF8 (3)
- FIBRONECTIN (3)
- GABARAPL1 (3)
- GADD45B (3)
- GATA3 (3)
- GFI1 (3)
- GLUTATHIONE TRANSFERASE ZETA 1 (3)
- HAO1 (3)
- IL17 (3)
- IL17F (3)
Tags
- N-His (2369)
- N-GST and C-His (435)
- Tag Free (335)
- C-His (235)
- N-GST and C-HIS (150)
- N-GST (55)
- N-His-ABP (Albumin-Binding Protein) (52)
- His-tag (46)
- N-His&C-His (22)
- N-His-Trx (18)
- N-GST, C-His (13)
- N-T7&C-His (12)
- N-Trx&His (12)
- Tag-free (7)
- N-6His (6)
- N-His PDCD1(Pro21-Val170) (5)
- N-GST&His (4)
- N-HIS (4)
- N-His&SUMO (4)
- N-His-GST (4)
- C-Avi&6His (3)
- C-HIS (3)
- N-Avi (3)
- Myc-DDK (2)
- N-His&C-Fc (2)
- N-His&T7 (2)
- N-His-PDCD1(Pro21-Val170) (2)
- N-MARI (2)
- N-Trx (2)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- C-HIS/C (1)
- N-AVI (1)
- N-DDK (1)
- N-FUSION (1)
- N-GFP (1)
- N-GST(CKM) and N-His(CKB) (1)
- N-T7 (1)
- N-TRX (1)
- N-TRXA (1)
- N-Trx-His (1)
Families
- Druggable Genome (1838)
- Transcription Factors (595)
- Secreted Protein (570)
- Transmembrane (548)
- ES Cell Differentiation/IPS (206)
- Protein Kinase (149)
- Stem cell - Pluripotency (136)
- Protease (107)
- Adult stem cells (73)
- Embryonic stem cells (61)
- Phosphatase (49)
- Induced pluripotent stem cells (35)
- Ion Channels: Other (30)
- Cancer stem cells (29)
- Stem cell relevant signaling - TGFb/BMP signaling pathway (24)
- Stem cell relevant signaling - JAK/STAT signaling pathway (19)
- Stem cell relevant signaling - Wnt Signaling pathway (15)
- 10 mM Tris-HCl (12)
- GPCR (12)
- Nuclear Hormone Receptor (12)
- the solution can be further dialyzed against PBS (phosphate buffered saline (12)
- Stem cell relevant signaling - DSL/Notch pathway (11)
- 2 mM dithiothreitol (10)
- e.g. Dulbecco's PBS). (10)
-
ELISA.
Western Blot. (9) - Ion Channels: Potassium (8)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (8)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (6) - P450 (6)
- pH 7.4). For immunization purposes (6)
- Ion Channels: Transient receptor potential (4)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (4) -
pH 7.4).
For immunization purposes (4) -
pH 7.4).
For immunization purposes (4) - Ion Channels: Cys-loop Receptors (3)
- Western Blot. (3)
- 0.1% (w/v) insulin (2)
- 10mM Tris-HCl (2)
- 1mM DTT (2)
- 2 mM dithiothreitol (2)
-
2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes (2) - 2mM dithiothreitol (2)
- 63mM sodium phosphate (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - Can be used as standard in a Sandwich ELISA. (2)
- Ion Channels: Sodium (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above) (2) -
Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris (2) -
both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2) - e.g. Dulbeccos PBS). (2)
- e.g.Dulbecco's PBS). (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
-
ELISA.
Western blotting. (1) -
Positive control for Western blot analysis.
Standard for ELISA. (1) -
Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T (1) -
Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A (1) - Forssmann WG (1)
- Hawkins JA (1)
- Hidaka Y (1)
- Ion Channels: ATP Receptors (1)
- Marx UC (1)
-
Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA (1) - Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- Neudecker P (1)
- Nourse A (1)
- Rosch P (1)
- Schulz A (1)
- Shimonishi Y (1)
-
Western Blot.
Protocol: 1. Lauber T (1) - Western Blotting. (1)
Pathways
- Metabolic pathways (256)
- Cytokine-cytokine receptor interaction (241)
- Pathways in cancer (236)
- MAPK signaling pathway (166)
- Jak-STAT signaling pathway (110)
- Hematopoietic cell lineage (98)
- Focal adhesion (96)
- Regulation of actin cytoskeleton (88)
- Chemokine signaling pathway (83)
- Melanoma (79)
- Toll-like receptor signaling pathway (75)
- Apoptosis (73)
- T cell receptor signaling pathway (71)
- Natural killer cell mediated cytotoxicity (69)
- Alzheimer's disease (66)
- NOD-like receptor signaling pathway (66)
- Systemic lupus erythematosus (64)
- TGF-beta signaling pathway (59)
- Wnt signaling pathway (58)
- Prostate cancer (57)
- Neurotrophin signaling pathway (56)
- Fc epsilon RI signaling pathway (54)
- Hypertrophic cardiomyopathy (HCM) (53)
- Cell cycle (51)
- Cytosolic DNA-sensing pathway (51)
- ErbB signaling pathway (51)
- Huntington's disease (51)
- Ubiquitin mediated proteolysis (49)
- Graft-versus-host disease (47)
- Type I diabetes mellitus (46)
- Glioma (45)
- Parkinson's disease (45)
- Renal cell carcinoma (45)
- Dilated cardiomyopathy (44)
- Oocyte meiosis (44)
- Amyotrophic lateral sclerosis (ALS) (43)
- Melanogenesis (43)
- Pancreatic cancer (43)
- p53 signaling pathway (43)
- Colorectal cancer (42)
- RIG-I-like receptor signaling pathway (41)
- Insulin signaling pathway (40)
- Adipocytokine signaling pathway (39)
- Hedgehog signaling pathway (39)
- Prion diseases (39)
- Acute myeloid leukemia (38)
- Small cell lung cancer (38)
- Chronic myeloid leukemia (37)
- mTOR signaling pathway (37)
- Asthma (36)
- Endocytosis (36)
- Bladder cancer (35)
- Gap junction (34)
- Ribosome (34)
- Allograft rejection (33)
- Pyrimidine metabolism (33)
- B cell receptor signaling pathway (32)
- Basal cell carcinoma (32)
- PPAR signaling pathway (32)
- Leukocyte transendothelial migration (31)
- Progesterone-mediated oocyte maturation (31)
- Purine metabolism (30)
- VEGF signaling pathway (30)
- Calcium signaling pathway (29)
- GnRH signaling pathway (28)
- Base excision repair (27)
- Type II diabetes mellitus (27)
- Antigen processing and presentation (26)
- Adherens junction (25)
- Axon guidance (25)
- Arginine and proline metabolism (24)
- Autoimmune thyroid disease (24)
- Endometrial cancer (24)
- Epithelial cell signaling in Helicobacter pylori infection (24)
- Long-term potentiation (24)
- Regulation of autophagy (24)
- Nitrogen metabolism (23)
- Oxidative phosphorylation (23)
- Vascular smooth muscle contraction (23)
- Cell adhesion molecules (CAMs) (22)
- Glycolysis / Gluconeogenesis (22)
- Spliceosome (22)
- Complement and coagulation cascades (21)
- Neuroactive ligand-receptor interaction (21)
- ECM-receptor interaction (20)
- Long-term depression (19)
- Fc gamma R-mediated phagocytosis (18)
- Non-small cell lung cancer (18)
- Primary immunodeficiency (18)
- Tyrosine metabolism (18)
- Arrhythmogenic right ventricular cardiomyopathy (ARVC) (16)
- Drug metabolism - other enzymes (15)
- Glutathione metabolism (15)
- Glycerophospholipid metabolism (15)
- Metabolism of xenobiotics by cytochrome P450 (15)
- Pathogenic Escherichia coli infection (15)
- Phenylalanine metabolism (15)
- Porphyrin and chlorophyll metabolism (15)
- Tryptophan metabolism (15)
- Cardiac muscle contraction (14)
- Fructose and mannose metabolism (14)
- Glycine (14)
- RNA degradation (14)
- SNARE interactions in vesicular transport (14)
- serine and threonine metabolism (14)
- Amino sugar and nucleotide sugar metabolism (13)
- Cysteine and methionine metabolism (13)
- Mismatch repair (13)
- Nucleotide excision repair (13)
- Olfactory transduction (13)
- Thyroid cancer (13)
- Tight junction (13)
- ABC transporters (12)
- Alanine (12)
- Viral myocarditis (12)
- aspartate and glutamate metabolism (12)
- Homologous recombination (11)
- Notch signaling pathway (11)
- Retinol metabolism (11)
- Sulfur metabolism (11)
- Aminoacyl-tRNA biosynthesis (10)
- DNA replication (10)
- Dorso-ventral axis formation (10)
- Drug metabolism - cytochrome P450 (10)
- Pyruvate metabolism (10)
- Arachidonic acid metabolism (9)
- Basal transcription factors (9)
- Glyoxylate and dicarboxylate metabolism (9)
- Inositol phosphate metabolism (9)
- Lysosome (9)
- Maturity onset diabetes of the young (9)
- Phosphatidylinositol signaling system (9)
- Androgen and estrogen metabolism (8)
- Citrate cycle (TCA cycle) (8)
- Terpenoid backbone biosynthesis (8)
- Valine (8)
- Butanoate metabolism (7)
- Ether lipid metabolism (7)
- Fatty acid metabolism (7)
- Lysine degradation (7)
- N-Glycan biosynthesis (7)
- Pantothenate and CoA biosynthesis (7)
- Propanoate metabolism (7)
- Proteasome (7)
- leucine and isoleucine degradation (7)
- One carbon pool by folate (6)
- Renin-angiotensin system (6)
- Selenoamino acid metabolism (6)
- Ubiquinone and other terpenoid-quinone biosynthesis (6)
- Vibrio cholerae infection (6)
- beta-Alanine metabolism (6)
- Galactose metabolism (5)
- Glycosphingolipid biosynthesis - lacto and neolacto series (5)
- Heparan sulfate biosynthesis (5)
- Methane metabolism (5)
- Nicotinate and nicotinamide metabolism (5)
- Pentose and glucuronate interconversions (5)
- Pentose phosphate pathway (5)
- Riboflavin metabolism (5)
- Sphingolipid metabolism (5)
- Synthesis and degradation of ketone bodies (5)
- Chondroitin sulfate biosynthesis (4)
- Histidine metabolism (4)
- Keratan sulfate biosynthesis (4)
- Linoleic acid metabolism (4)
- Non-homologous end-joining (4)
- O-Glycan biosynthesis (4)
- Phenylalanine (4)
- Starch and sucrose metabolism (4)
- Steroid biosynthesis (4)
- Taste transduction (4)
- alpha-Linolenic acid metabolism (4)
- tyrosine and tryptophan biosynthesis (4)
- C21-Steroid hormone metabolism (3)
- Circadian rhythm - mammal (3)
- Folate biosynthesis (3)
- Primary bile acid biosynthesis (3)
- RNA polymerase (3)
- Caffeine metabolism (2)
- D-Arginine and D-ornithine metabolism (2)
- D-Glutamine and D-glutamate metabolism (2)
- Glycosaminoglycan degradation (2)
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis (2)
- Limonene and pinene degradation (2)
- Other glycan degradation (2)
- Taurine and hypotaurine metabolism (2)
- leucine and isoleucine biosynthesis (2)
- Ascorbate and aldarate metabolism (1)
- Biosynthesis of unsaturated fatty acids (1)
- Cyanoamino acid metabolism (1)
- Fatty acid elongation in mitochondria (1)
- Glycerolipid metabolism (1)
- Glycosphingolipid biosynthesis - ganglio series (1)
- Lipoic acid metabolism (1)
- Protein export (1)
