Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

Over-expression Lysates

CytoSections

Mass Spec Standards

Mutant ORF Clones

Human

Mouse

PPP2R2B

PTEN

PTPN20B

GLEPP1

EYA1

PPM1B

PPP2R4

PPP2R5C

PTPN5

CDC25B

PTPRS

SIRP ALPHA

MTMR14

PDP1

SKIP

ACID PHOSPHATASE

DUSP13

PPP2R5D

SHP1

PPM1A

PPP1R7

PPP2R1B

PPP3CB

PTPDC1

CTDSP1

MTMR2

PPM1G

PTP ALPHA

PTPH1

SYNAPTOJANIN

PTPRD

CDC25C

PCPTP1

PTP4A3

DUSP4

PTPN7

EYA4

STYX

TCPTP

DUSP15

PPP6C

PTP EPSILON

PTP KAPPA

PTP4A2

SET

PPEF1

PPP1A

PPP2R2C

PTPN12

CTDSPL

DUSP19

PROTEIN PHOSPHATASE 1 BETA

DEP1

DUSP10

PTPN18

PTPRN2

CD45

CDC25A

DUSP6

PP1C GAMMA

PPP2R3A

PROSTATIC ACID PHOSPHATASE

CDC14A

HCE

PLASTICITY RELATED GENE 3

PPP2R5E

PPP3CC

CDKN3

DUSP3

LIPRIN ALPHA 1

PP2A-BETA

PTP1B

PTPN22

PTPRB

SLINGSHOT HOMOLOG 1

CALCINEURIN A

CDC14B

MTMR3

PHOSPHOSERINE PHOSPHATASE

PPP1R1C

PPP2R2D

PTP4A1

DUSP22

PP4R1

PPM1D

PPM1F

PPP1R9B

PPP2R2A

PRY

DUSP23

EYA3

ILKAP

MTMR1

PNK

PPP2R1A

SHP2

SSH2

SYNAPTOJANIN 2

DAPP1

DUSP14

WB

IHC

HEK293T

E. coli

HEK293

Sf9

Plasmids

Myc-DDK

C-Myc/DDK

Tag Free

mGFP

TurboGFP

C-MYC/DDK

His-tag

C-His

N-His

N-GST and C-His

N-His&C-His

C-6His

C-6His-Avi

C-DDK

C-DDK/His

C-FLAG

N-HIS

N-His-ABP (Albumin-Binding Protein)

N-His-SUMO

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

WB

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

Induced pluripotent stem cells

P450

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

ELISA.
Western Blot.

FACS

Suitable for use in ELISA.

ELISA.

ELISA.

ELISA.
Western blot.
Lateral flow.

Immunogen

Secreted

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

000 or less. This should be performed in a cold room.

1 mM DTT

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

RIA.
Western blot.

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Apo AI can be renatured into PBS

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA and Western blot.

ELISA. Can be used for all immunological/laboratory methods requiring highly purified antigens.

ELISA.
Western blot.

ELISA: 0.4-0.8 µg/well.
After thawing

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Second order rate constants for inhibition of:
uPA = 6.3 x 10e6 M-1s-1
tPA = 5.7 x 10e6 M-1s-1

Sodium citric aci d 0.8g

Specific methodologies have not been tested using this product.

Suitable for ELISA and Western blot.

Suitable for use in ELISA and Western blot.

Suitable for use in Western blot.

Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces

TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14

VICTOR X3)

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

calibrator in immunoassays and in vitro studies.

calibrator in immunoassays and in vitro studies.

completely thaw the tube at 37°C without agitation

e.g. Dulbecco's PBS).

flicking or mixing

glassware and microcarrier beads. After spin and opening of the vial

including plasticware

mix thoroughly by vortexing before use!

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

then gently mix the contents with a pipette.

MAPK signaling pathway

Oocyte meiosis

Wnt signaling pathway

Tight junction

Metabolic pathways

Long-term potentiation

T cell receptor signaling pathway

Insulin signaling pathway

Adherens junction

Natural killer cell mediated cytotoxicity

B cell receptor signaling pathway

Inositol phosphate metabolism

Phosphatidylinositol signaling system

Riboflavin metabolism

Focal adhesion

Cell cycle

Regulation of actin cytoskeleton

Alzheimer's disease

Amyotrophic lateral sclerosis (ALS)

Apoptosis

Axon guidance

Calcium signaling pathway

VEGF signaling pathway

Progesterone-mediated oocyte maturation

Long-term depression

TGF-beta signaling pathway

p53 signaling pathway

Fructose and mannose metabolism

Thiamine metabolism

Vascular smooth muscle contraction

Endometrial cancer

Glioma

Melanoma

Pathways in cancer

Prostate cancer

Small cell lung cancer

Jak-STAT signaling pathway

Cell adhesion molecules (CAMs)

Epithelial cell signaling in Helicobacter pylori infection

Fc gamma R-mediated phagocytosis

Primary immunodeficiency

Type I diabetes mellitus

Glycine

serine and threonine metabolism

Adipocytokine signaling pathway

Chronic myeloid leukemia

Leukocyte transendothelial migration

Neurotrophin signaling pathway

Renal cell carcinoma

Biosynthesis of unsaturated fatty acids

Sphingolipid metabolism

E. coli

Human

Human 293

Neomycin

Puromycin

None

1

2

Yes

In Stock