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MAP3K4 CRISPRa kit - CRISPR gene activation of human mitogen-activated protein kinase kinase kinase 4
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti-ORF clone of MAP3K4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 2
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Transient overexpression lysate of mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
MAP3K4 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
(untagged)-Human cDNA FLJ37310 fis, clone BRAMY2016706
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
MAP3K4 (untagged)-Human mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Neo |
MAP3K4 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
3' UTR clone of mitogen-activated protein kinase kinase kinase 4 (MAP3K4) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
MAP3K4 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
MAP3K4 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
MAP3K4 (untagged)-Human mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
MAP3K4 (untagged) - Human mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
3' UTR clone of mitogen-activated protein kinase kinase kinase 4 (MAP3K4) transcript variant 2 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
MAP3K4 (untagged) - Human mitogen-activated protein kinase kinase kinase 4 (MAP3K4), transcript variant 5
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qSTAR qPCR primer pairs against Homo sapiens gene MAP3K4
