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S100A1 - human gene knockout kit via CRISPR, HDR mediated
| Format | 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control |
| Donor DNA | Luciferase-Puro |
Products
S100A1 CRISPRa kit - CRISPR gene activation of human S100 calcium binding protein A1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
S100A1 MS Standard C13 and N15-labeled recombinant protein (NP_006262)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
S100A1 (untagged)-Human S100 calcium binding protein A1 (S100A1)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
S100A1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene S100A1
| Application | Plasmid of exact quantity for transcript copy number calculation |
S100A1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
S100A1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of S100A1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
qSTAR qPCR primer pairs against Homo sapiens gene S100A1
3' UTR clone of S100 calcium binding protein A1 (S100A1) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
