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SESN2 CRISPRa kit - CRISPR gene activation of human sestrin 2
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
SESN2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
SESN2 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
SESN2 MS Standard C13 and N15-labeled recombinant protein (NP_113647)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
qPCR primer pairs and template standards against Homo sapiens gene SESN2
| Application | Plasmid of exact quantity for transcript copy number calculation |
3' UTR clone of sestrin 2 (SESN2) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
SESN2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
qSTAR qPCR primer pairs against Homo sapiens gene SESN2
Transient overexpression of SESN2 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
