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USP25 CRISPRa kit - CRISPR gene activation of human ubiquitin specific peptidase 25
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
USP25 MS Standard C13 and N15-labeled recombinant protein (NP_037528)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
USP25 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of ubiquitin specific peptidase 25 (USP25)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
USP25 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene USP25
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene USP25
| Application | Plasmid of exact quantity for transcript copy number calculation |
USP25 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
USP25 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
USP25 (untagged) - Human ubiquitin specific peptidase 25 (USP25), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
USP25 (untagged) - Human ubiquitin specific peptidase 25 (USP25), transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
3' UTR clone of ubiquitin specific peptidase 25 (USP25) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene USP25
Transient overexpression of USP25 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
