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Ccnd3 CRISPRa kit - CRISPR gene activation of mouse cyclin D3
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Cyclin D3 (CCND3) mouse monoclonal antibody, clone DCS-2.2, Purified
| Applications | IHC, IP, WB |
| Reactivities | Human, Monkey, Mouse, Rat |
CCND3 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Ccnd3 (untagged) - Mouse cyclin D3 (Ccnd3), transcript variant 1, (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
Ccnd3 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Ccnd3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
qSTAR qPCR primer pairs against mus musculus gene Ccnd3
qPCR primer pairs and template standards against Homo sapiens gene CCND3
| Application | Plasmid of exact quantity for transcript copy number calculation |
3' UTR clone of cyclin D3 (CCND3) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
3' UTR clone of cyclin D3 (CCND3) transcript variant 4 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Ccnd3 (untagged) - Mouse cyclin D3 (Ccnd3), transcript variant 3
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
CCND3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Ccnd3 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Ccnd3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Ccnd3 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
