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RFNG CRISPRa kit - CRISPR gene activation of human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti-ORF clone of RFNG (Myc-DDK-tagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
RFNG (untagged)-Human RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
Rfng (untagged ORF) - Rat RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Rfng), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qPCR primer pairs and template standards against Homo sapiens gene RFNG
| Application | Plasmid of exact quantity for transcript copy number calculation |
Transient overexpression lysate of RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
RFNG - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
RFNG - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Rfng - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Rfng - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
RFNG HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Rfng - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Rfng - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Rfng (untagged) - Mouse RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Rfng), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
3' UTR clone of RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (RFNG) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
