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PPM1B CRISPRa kit - CRISPR gene activation of human protein phosphatase, Mg2+/Mn2+ dependent 1B
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti ORF clone of Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 1, Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti-ORF clone of PPM1B (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 4
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti-ORF clone of PPM1B (Myc-DDK-tagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 5
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
PPM1B HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform (PPM1B), transcript variant 2
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform (PPM1B), transcript variant 1
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
PPM1B (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
PPM1B MS Standard C13 and N15-labeled recombinant protein (NP_808907)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
PPM1B MS Standard C13 and N15-labeled recombinant protein (NP_002697)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
PPM1B MS Standard C13 and N15-labeled recombinant protein (NP_808908)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 3
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-AC |
qPCR primer pairs and template standards against Homo sapiens gene PPM1B
| Application | Plasmid of exact quantity for transcript copy number calculation |
3' UTR clone of protein phosphatase 1B (formerly 2C) magnesium-dependent beta isoform (PPM1B) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
