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Cytokeratin 14 (KRT14) mouse monoclonal antibody, clone DE-SPK14, Purified
| Applications | IHC, WB |
| Reactivities | Human |
Products
qSTAR qPCR primer pairs against Mus musculus gene Krt14
qPCR primer pairs and template standards against Homo sapiens gene KRT14
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Mus musculus gene Krt14
| Application | Plasmid of exact quantity for transcript copy number calculation |
Krt14 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Krt14 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
KRT14 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Krt14 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Krt14 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Cytokeratin 14 human recombinant protein, 0.25 mg
| Expression Host | E. coli |
qSTAR qPCR primer pairs against Homo sapiens gene KRT14
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Species
Reactivities
Antibody Hosts
Expression Hosts
Lentivirus
Vectors
Conjugates
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Clonalities
Mutants
Assay Types
Brands
Families
- 10 mM Tris-HCl (1)
- 2 mM dithiothreitol (1)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (1) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (1)
- e.g. Dulbecco's PBS). (1)
-
pH 7.4).
For immunization purposes (1) - the solution can be further dialyzed against PBS (phosphate buffered saline (1)
