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UAP1 CRISPRa kit - CRISPR gene activation of human UDP-N-acetylglucosamine pyrophosphorylase 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
UAP1 (untagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
UAP1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
UAP1 MS Standard C13 and N15-labeled recombinant protein (NP_003106)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
Uap1 (untagged) - Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (cDNA clone MGC:28907 IMAGE:4920702), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
qPCR primer pairs and template standards against Homo sapiens gene UAP1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Purified recombinant protein of Mouse UDP-N-acetylglucosamine pyrophosphorylase 1 (Uap1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
UAP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Uap1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Uap1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Uap1 (untagged) - Rat UDP-N-acteylglucosamine pyrophosphorylase 1 (Uap1)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
3' UTR clone of UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Transient overexpression of UAP1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Uap1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene UAP1
