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ATP6V1B1 CRISPRa kit - CRISPR gene activation of human ATPase H+ transporting V1 subunit B1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Atp6v1b1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
qSTAR qPCR primer pairs against Homo sapiens gene ATP6V1B1
qPCR primer pairs and template standards against Mus musculus gene Atp6v1b1
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene ATP6V1B1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Atp6v1b1 (untagged ORF) - Rat ATPase, H transporting, lysosomal V1 subunit B1 (Atp6v1b1), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
ATP6V1B1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Atp6v1b1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Atp6v1b1 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
ATP6V1B1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Transient overexpression of ATP6V1B1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
Atp6v1b1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
3' UTR clone of ATPase H+ transporting lysosomal 56/58kDa V1 subunit B1 (ATP6V1B1) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Mus musculus gene Atp6v1b1
Purified recombinant protein of Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
