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Eif4b CRISPRa kit - CRISPR gene activation of mouse eukaryotic translation initiation factor 4B
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
Lenti ORF clone of Eif4b (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4B (Eif4b), (10 ug)
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Transient overexpression lysate of eukaryotic translation initiation factor 4B (EIF4B)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
EIF4B (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Eif4b (untagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114), (10ug)
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | PCMV6-Kan/Neo |
EIF4B MS Standard C13 and N15-labeled recombinant protein (NP_001408)
| Tag | C-Myc/DDK |
| Expression Host | HEK293 |
EIF4B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
EIF4B - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
qPCR primer pairs and template standards against Homo sapiens gene EIF4B
| Application | Plasmid of exact quantity for transcript copy number calculation |
EIF4B (untagged) - Human eukaryotic translation initiation factor 4B (EIF4B), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Eif4b (untagged ORF) - Rat eukaryotic translation initiation factor 4B (Eif4b), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Purified recombinant protein of Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
EIF4B - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Eif4b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Eif4b - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
