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Rabbit monoclonal to Vimentin
| Applications | IHC, WB |
| Reactivities | Human |
Products
Anti-Vimentin Antibody
| Applications | IHC, WB |
| Reactivities | Human |
Anti-Vimentin Antibody
| Applications | ICC, WB |
| Reactivities | Human, Rat, Mouse, Chicken |
Vimentin (VIM) mouse monoclonal antibody, clone 65E, Purified
| Applications | IHC, WB |
| Reactivities | Human |
VIMENTIN Mouse Monoclonal Antibody
| Applications | IHC |
| Reactivities | Human |
Vimentin (VIM) chicken polyclonal antibody
| Applications | IHC |
| Reactivities | Rabbit |
Vimentin (VIM) rabbit polyclonal antibody, Purified
| Applications | IHC |
| Reactivities | Human |
Vimentin (VIM) rabbit polyclonal antibody, Purified
| Applications | IHC |
| Reactivities | Human |
qPCR primer pairs and template standards against Homo sapiens gene VIM
| Application | Plasmid of exact quantity for transcript copy number calculation |
Vim - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Vim - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Vim (untagged ORF) - Rat vimentin (Vim), (10 ug)
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
Vim (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Vimentin human recombinant protein, 0.25 mg
| Expression Host | E. coli |
Purified recombinant protein of Mouse vimentin (Vim), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
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Knockout (KO) Validation
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- e.g. Dulbecco's PBS). (4)
- 10 mM sodium phosphate (2)
- 10mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
- 2mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) -
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above) (2) - pH 7.4). For immunization purposes (2)
-
pH 7.4).
For immunization purposes (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
