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ACAN CRISPRa kit - CRISPR gene activation of human aggrecan
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Aggrecan/ACAN with <50pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Aggrecan/ACAN tissue specificity: Restricted to cartilages. .
| Type | Sandwich ELISA kit of Quantitative Detection for Human Aggrecan |
| Format | 8x12 divisible strips |
| Reactivity | Human |
For quantitative detection of human Aggrecan in cell culture supernates, serum and urine.
| Type | Sandwich ELISA kit of Quantitative Detection for Human Aggrecan |
| Format | 8x12 divisible strips |
| Reactivity | Human |
ACAN (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene ACAN
3' UTR clone of aggrecan (ACAN) transcript variant 2 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
ACAN - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
ACAN - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
3' UTR clone of aggrecan (ACAN) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
