Products

DCTN2 CRISPRa kit - CRISPR gene activation of human dynactin subunit 2

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Dynactin subunit 2 (1-403, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

Dynactin subunit 2 (1-406, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

Dynactin subunit 2 (1-403, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

Dynactin subunit 2 (1-401, His-tag) human recombinant protein, 50 µg

Tag His-tag
Expression Host E. coli

Dynactin subunit 2 (1-406, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

Dynactin subunit 2 (1-401, His-tag) human recombinant protein, 0.25 mg

Tag His-tag
Expression Host E. coli

qPCR primer pairs and template standards against Homo sapiens gene DCTN2

Application Plasmid of exact quantity for transcript copy number calculation

DCTN2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

3' UTR clone of dynactin 2 (p50) (DCTN2) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

DCTN2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).