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DNAH5 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
DNAH5 (Myc-DDK-tagged)-Human dynein, axonemal, heavy chain 5 (DNAH5)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pRMT-tGFP, Promoter-less GFP Reporter Vector |
- TrueORF®
DNAH5 (tGFP-tagged) - Human dynein, axonemal, heavy chain 5 (DNAH5)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
DNAH5 CRISPRa kit - CRISPR gene activation of human dynein axonemal heavy chain 5
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
DNAH5 (untagged)-Human dynein, axonemal, heavy chain 5 (DNAH5)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
DNAH5 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
DNAH5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
DNAH5 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
3' UTR clone of dynein axonemal heavy chain 5 (DNAH5) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
Recombinant Human DNAH5 Protein(N-His-SUMO)
| Tag | N-His-SUMO |
qSTAR qPCR primer pairs against Homo sapiens gene DNAH5
