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MAPKAPK5 CRISPRa kit - CRISPR gene activation of human MAPK activated protein kinase 5
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
MAPKAPK5 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
MAPKAPK5 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
MAPKAPK5 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
qSTAR qPCR primer pairs against Homo sapiens gene MAPKAPK5
qPCR primer pairs and template standards against Homo sapiens gene MAPKAPK5
| Application | Plasmid of exact quantity for transcript copy number calculation |
MAPKAPK5 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
3' UTR clone of mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
3' UTR clone of mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5) transcript variant 2 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
