Products

PC CRISPRa kit - CRISPR gene activation of human pyruvate carboxylase

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

PC (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

qPCR primer pairs and template standards against Homo sapiens gene PC

Application Plasmid of exact quantity for transcript copy number calculation

3' UTR clone of pyruvate carboxylase (PC) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of pyruvate carboxylase (PC) nuclear gene encoding mitochondrial protein transcript variant 3 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

qPCR primer pairs and template standards against Homo sapiens gene PC

Application Plasmid of exact quantity for transcript copy number calculation

3' UTR clone of pyruvate carboxylase (PC) nuclear gene encoding mitochondrial protein transcript variant 2 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

PC (untagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 2

Type Human Untagged Clone
Tag Tag Free
Vector pCMV6 series

qSTAR qPCR primer pairs against Homo sapiens gene PC