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POLA2 (Myc-DDK-tagged)-Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
POLA2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
POLA2 (tGFP-tagged) - Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
POLA2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Lenti ORF clone of Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2), mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Purified recombinant protein of Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
| Tag | N-GST and C-His |
| Expression Host | E. coli |
POLA2 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
Transient overexpression lysate of polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
POLA2 CRISPRa kit - CRISPR gene activation of human DNA polymerase alpha 2, accessory subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
POLA2 (untagged)-Human polymerase (DNA directed), alpha 2 (70kD subunit) (POLA2)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
qPCR primer pairs and template standards against Homo sapiens gene POLA2
| Application | Plasmid of exact quantity for transcript copy number calculation |
POLA2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
POLA2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
3' UTR clone of polymerase (DNA directed) alpha 2 (70kD subunit) (POLA2) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
