POLE3 CRISPRa kit - CRISPR gene activation of human DNA polymerase epsilon 3, accessory subunit
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
POLE3 CRISPRa kit - CRISPR gene activation of human DNA polymerase epsilon 3, accessory subunit
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
POLE3 (1-147, His-tag) human protein, 0.1 mg
Tag | His-tag |
Expression Host | E. coli |
POLE3 (1-147, His-tag) human protein, 0.5 mg
Tag | His-tag |
Expression Host | E. coli |
POLE3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |
POLE3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
3' UTR clone of polymerase (DNA directed) epsilon 3 (p17 subunit) (POLE3) for miRNA target validation
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
POLE3 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene POLE3