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PPP1R3D - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
PPP1R3D (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
PPP1R3D (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti-ORF clone of PPP1R3D (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
PPP1R3D CRISPRa kit - CRISPR gene activation of human protein phosphatase 1 regulatory subunit 3D
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Lenti-ORF clone of PPP1R3D (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
PPP1R3D (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
qPCR primer pairs and template standards against Homo sapiens gene PPP1R3D
| Application | Plasmid of exact quantity for transcript copy number calculation |
PPP1R3D - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
PPP1R3D - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
PPP1R3D (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene PPP1R3D
