Products

SMS CRISPRa kit - CRISPR gene activation of human spermine synthase

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Spermine synthase (SMS) (1-366, His-tag) human recombinant protein, 0.25 mg

Tag His-tag
Expression Host E. coli

Spermine synthase (SMS) (1-366, His-tag) human recombinant protein, 50 µg

Tag His-tag
Expression Host E. coli

qPCR primer pairs and template standards against Homo sapiens gene SMS

Application Plasmid of exact quantity for transcript copy number calculation

3' UTR clone of spermine synthase (SMS) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

SMS (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).