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qSTAR qPCR primer pairs against Homo sapiens gene VIM
Products
Vimentin human recombinant protein, 0.1 mg
| Expression Host | E. coli |
VIM (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SR322185 is the updated version of SR305078.
VIM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
VIM - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
VIM CRISPRa kit - CRISPR gene activation of human vimentin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene VIM
| Application | Plasmid of exact quantity for transcript copy number calculation |
Vimentin human recombinant protein, 0.25 mg
| Expression Host | E. coli |
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- 10mM Tris-HCl (2)
- 2mM dithiothreitol (2)
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Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above) (2) - e.g. Dulbecco's PBS). (2)
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pH 7.4).
For immunization purposes (2) - protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl (2)
- the solution can be further dialyzed against PBS (Phosphate Buffered Saline (2)
