qSTAR qPCR primer pairs against Homo sapiens gene YWHAE
Products
YWHAE CRISPRa kit - CRISPR gene activation of human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
14-3-3 protein epsilon (1-255) human recombinant protein, 0.5 mg
Expression Host | E. coli |
14-3-3 protein epsilon (1-255) human recombinant protein, 0.1 mg
Expression Host | E. coli |
YWHAE - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
YWHAE (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene YWHAE
Application | Plasmid of exact quantity for transcript copy number calculation |
YWHAE - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |
3' UTR clone of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon polypeptide (YWHAE) transcript variant 1 for miRNA target validation
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |