qSTAR qPCR primer pairs against Mus musculus gene Actb
Products
Actb (Myc-DDK-tagged) - Mouse actin, beta (Actb)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
- TrueORF®
Actb (tGFP-tagged) - Mouse actin beta (Actb), (10ug)
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
- TrueORF®
Actb - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
Actb (untagged) - Mouse actin, beta (Actb), (10ug)
Type | Mouse Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
Lenti ORF clone of Actb (mGFP-tagged) - Mouse actin, beta (Actb)
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
Lenti ORF clone of Actb (Myc-DDK-tagged) - Mouse actin, beta (Actb)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Actb CRISPRa kit - CRISPR gene activation of mouse actin, beta
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Actb (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Mus musculus gene ACTB NM_007393
Application | Plasmid of exact quantity for transcript copy number calculation |
Actb - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
Purified recombinant protein of Mouse actin, beta (Actb), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Tag | C-MYC/DDK |
Expression Host | HEK293T |
Actb - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |