Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
- TrueORF®
Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
Sema4b - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
Sema4b (tGFP-tagged) - Mouse sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B (Sema4b), (10ug)
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
Lenti ORF clone of Sema4b (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Vector | pLenti-C-mGFP-P2A-Puro |
Lenti ORF clone of Sema4b (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
Sema4b CRISPRa kit - CRISPR gene activation of mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Sema4b (untagged) - Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), (10ug)
Type | Mouse Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
Purified recombinant protein of Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B (Sema4b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Tag | C-MYC/DDK |
Expression Host | HEK293T |
Sema4b - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
Sema4b - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |
qSTAR qPCR primer pairs against Mus musculus gene Sema4b
Sema4b (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |