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OriGene联合CELL杂志共同出版的
CRISPR论文集
Video overview of cDNA clone
offering
Video brochure for cDNA clone
OriGene cDNA clones in recent publications
Epilepsy-associated GRIN2A mutations reduce NMDA receptor trafficking and agonist potency - molecular profiling and functional rescue Sci Rep 2017 [GRIN2A]

Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17 Oncogene 2017 [PTH1R]

Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development. Sci Rep 2017 [GPATCH3]

Ziploc-ing the Structure 2.0: endoplasmic reticulum resident peptidyl prolyl isomerases show different activities toward hydroxyproline J. Biol. Chem. 2017 [FKBP2]

穿梭表达载体

相同的ORF插入片段,可构建于多种不同载体,适用于各种应用。

所有TrueORF插入片段构建在pCMV6通用载体中,只通过一个简单的“剪切和粘贴”过程,就可很方便的通过穿梭载体系统更换相关载体,以用于各种下游蛋白研究。

目的载体提供以下选择:

  • 抗原决定簇标签,以用于蛋白纯化检测。
  • 荧光蛋白标签,以用于蛋白观察。
  • 多种药物筛选标记,用于建立稳定的细胞系。
  • 诱导表达(pTUNE)
  • E.coli 表达载体
载体订购或载体更换选项。

Entry VectorSKUC-TagE. coli
Selection
Cell
Selection
ExpressionPrice
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ugPS100001Myc-DDKKanamycinNeomycinMammalian¥3420
pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ugPS100063Myc-DDKKanamycinNeomycinMammalian¥3420

Lentiviral Vectors
New in 2013: OriGene now offers multiple Lentiviral vectors. Easy shuttling of TrueORF insert. Click the Lentiviral tab below

载体用途:

Epitope tagging FP tagging Selection markers Lenti / AAVNew Bacterial expression
Destination VectorSKUFP FeatureC-TagN-TagE. coli
Selection
Cell
Selection
Price
pCMV6-AC-GFPPS100010Green, dimerTurboGFP AmpicillinNeomycin¥5320
pCMV6-AN-GFPPS100019Green, dimer TurboGFPAmpicillinNeomycin¥5320
pCMV6-A-GFPPS100026Green, dimer  Ampicillin ¥5320
pCMV6-AC-IRES-GFPPS100027Green, dimerMyc-DDK AmpicillinNeomycin¥5320
pCMV6-AN-RFPPS100033Red,dimer TurboRFPAmpicillinNeomycin¥5320
pCMV6-AC-RFPPS100034Red,dimerTurboRFP AmpicillinNeomycin¥5320
pCMV6-AN-YFPPS100035Yellow, dimer TurboYFPAmpicillinNeomycin¥5320
pCMV6-AC-YFPPS100036Yellow, dimerTurboYFP AmpicillinNeomycin¥5320
pCMV6-AC-FP602PS100037Red, dimerTurboFP602 AmpicillinNeomycin¥5320
pCMV6-AC-FP635PS100038Far-red, dimerTurboFP635 AmpicillinNeomycin¥5320
pCMV6-AC-mKatePS100039Far-red, monomermKate AmpicillinNeomycin¥5320
pCMV6-AC-mGFPPS100040Green, monomermGFP AmpicillinNeomycin¥5320
pCMV6-AC-mRFPPS100041Red, monomermRFP AmpicillinNeomycin¥5320
pCMV6-AC-mYFPPS100042Yellow, monomermYFP AmpicillinNeomycin¥5320
pCMV6-AC-mBFPPS100043Blue, monomermBFP AmpicillinNeomycin¥5320
pCMV6-AC-mCFPPS100044Cyan, monomermCFP AmpicillinNeomycin¥5320
pCMV6-AN-FP602PS100045Red, dimer TurboFP602AmpicillinNeomycin¥5320
pCMV6-AN-FP635PS100046Far-red, dimer TurboFP635AmpicillinNeomycin¥5320
pCMV6-AN-mKatePS100047Far-red, monomer mKateAmpicillinNeomycin¥5320
pCMV6-AN-mGFPPS100048Green, monomer mGFPAmpicillinNeomycin¥5320
pCMV6-AN-mRFPPS100049Red, monomer mRFPAmpicillinNeomycin¥5320
pCMV6-AN-mYFPPS100050Yellow, monomer mYFPAmpicillinNeomycin¥5320
pCMV6-AN-mBFPPS100051Blue, monomer mBFPAmpicillinNeomycin¥5320
pCMV6-AN-mCFPPS100052Cyan, monomer mCFPAmpicillinNeomycin¥5320
pCMV6-AC-IRES-GFP-PuroPS100059Green, dimer  AmpicillinPuromycin¥5320
pCMV6-AN-GFP-C-HisPS100061Green, dimerHistGFPAmpicillinNeomycin¥6365
pLenti-C-GFPPS100065Green, dimerGFP Chloramphenicol ¥6365
pLenti-C-mGFPPS100071Green, MonomermGFP Chloramphenicol ¥6365
pLenti-EF1a-C-tGFPPS100072Green, dimertGFP Chloramphenicol ¥6365
pLenti-N-tGFP PS100073Green, dimer tGFPChloramphenicol ¥6365
pLenti-C-tRFPPS100074Red, dimertRFP Chloramphenicol ¥6365
pLenti-N-tRFPPS100077Red, dimer tRFPChloramphenicol ¥6365
pLenti-N-tYFPPS100078Yellow, dimer tYFPChloramphenicol ¥6365
pLenti-C-tYFPPS100079Yellow, dimertYFP Chloramphenicol ¥6365
pLenti-EF1a-C-mGFPPS100084Green, MonomermGFP Chloramphenicol ¥6365
pLenti-C-Myc-DDK-P2A-tGFPPS100088GFP, dimerMyc-DDK Chloramphenicol ¥6365
pAAV-AC-GFPPS100090GFP, dimertGFP ampicilin ¥5225
pAAV-AC-RFPPS100091RFP, dimertRFP ampicilin ¥5605
pLenti-C-mGFP-P2A-PuroPS100093Green, MonomermGFP ChloramphenicolPuromycin¥6745
pLenti-mRFP-P2A-PuroPS100094PuromycinmRFP ChloramphenicolPuromycin¥6745
pLenti-C-mGFP-P2A-BSDPS100102Green, monomermGFP ChloramphenicolBlasticidin¥6745

Photo gallery of the fluorescent-tagged proteins generated by OriGene with TrueORF clones

DDK-标签 (Flag®)的多肽序列:N-DYKDDDDK-C
Flag® 是Sigma-Aldrich公司注册的商标。

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