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OriGene cDNA clones in recent publications
Epilepsy-associated GRIN2A mutations reduce NMDA receptor trafficking and agonist potency - molecular profiling and functional rescue Sci Rep 2017 [GRIN2A]

Matrix metalloproteinase processing of PTHrP yields a selective regulator of osteogenesis, PTHrP1-17 Oncogene 2017 [PTH1R]

Whole-Exome Sequencing of Congenital Glaucoma Patients Reveals Hypermorphic Variants in GPATCH3, a New Gene Involved in Ocular and Craniofacial Development. Sci Rep 2017 [GPATCH3]

Ziploc-ing the Structure 2.0: endoplasmic reticulum resident peptidyl prolyl isomerases show different activities toward hydroxyproline J. Biol. Chem. 2017 [FKBP2]

pCMV6-AC-mKate, mammalian vector with C-terminal mKate tag, 10ug

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Catalog No. Description Price Delivery
PS100039 pCMV6-AC-mKate, mammalian vector with C-terminal mKate tag, 10ug ¥5480 现货
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特点:
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mKate2 tag.
  • mKate2 possesses fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at physiological pH 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date.
    mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time <20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues.
    mKate2 is mainly intended for protein labeling. Its far-red fluorescence allows easy and reliable separation from standard green fluorescent labels in dual-color high-throughput assays.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mKate2 -tagging vector, PS100047
  • Download full vector sequence
pCMV6-AC-mKate, mammalian vector with C-terminal mKate tag, 10ug Vector Image
多克隆位点示意图:
pCMV6-AC-mKate, mammalian vector with C-terminal mKate tag, 10ug MCS Image
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