OriGene Technologies, Inc.
  邮箱:  密码: 忘记密码?
注册 | 购物车 | 主页
搜索:    
About FL cDNA Clones RNAi Gene Expression Lysates and Proteins Antibodies Tissue IVD 原料 Other Products Tech Support
orange


OriGene proteins in recent publications

Fully functional proteins purified from transient TrueORF cDNA clone transfections.  Researchers purified GEN1 protein using anti-FLAG M2 affinity column and determined it resolved Holliday junction X0 efficiently in vitro, thus confirming GEN1 is human Holliday junction resolvase.

Identification of Holliday junction resolvases from humans and yeast, Stephen C. Y. Ip, Ulrich Rass, Miguel G. Blanco, Helen R. Flynn, J. Mark Skehel, Stephen C. West, Nature 456, 357 - 361 (20 Nov 2008), doi: 10.1038/nature07470,


View all citations

TNF (NM_000594) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP750007-1000 Recombinant protein of human Tumor Necrosis Factor-a (TNFa) produced in E. coli., 1 mg (Larger pack size?) ¥15000.00
¥3500.00
3-4 周
TA50011-100 4C5, Anti-DDK monoclonal antibody (100 μL) ¥1000 现货
Add to Shopping Cart



OriGene Data
Species:Human Expression Host:E. coli
Expression cDNA Clone or AA Sequence
A DNA sequence from TrueORF clone, RC206983, encoding the region (Val77-Leu233) of TNFa
Tag:Tag-free Predicted MW:17.5 kDa
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:Resuspend the protein to the desired concentration in proper buffer.
Buffer:Lyophilized from a sterile solution containing 20 mM phosphate buffer, PH7.2. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Endotoxin: <0.1 EU per 1 ?g of the protein by the LAL
BioActivity: Determined by the cytolysis of L929 cells in the presence of Actinomycin D. ED50 for this effect is typically 0.19-0.27 ng/ml.
Protein Families: Secreted Protein
Transmembrane
Transcription Factors
Druggable Genome
Protein Pathways: MAPK signaling pathway
Cytokine-cytokine receptor interaction
Apoptosis
TGF-beta signaling pathway
Toll-like receptor signaling pathway
NOD-like receptor signaling pathway
More Pathways >>
Protein Data
  Protein Image
 

Reference Data
RefSeq: NP_000585 RefSeq Size: 1669 RefSeq ORF: 702
LocusID: 7124 Cytogenetic: 6p21.33  
Synonyms : DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag? is a registered trademark of Sigma-Aldrich

 

bar
全国免费电话:400-6744-363(手机),800-9904-363(座机)订货邮箱:orderscn@origene.com,techsupport@origene.cn
电话:+86-10-59755312 传真:+86-10-59755322-888 地址:北京市经济技术开发区科创14街99号汇龙森科技园11栋
京ICP证07031721号 Copyright © 2018 北京傲锐东源(OriGene Technologies)生物科技有限公司,All Rights Reserved. 法律声明.