IFNG Mouse Monoclonal Antibody [Clone ID: 7B6]

Specifications

Product Data
Clone Name	7B6
Applications	FC, IHC, WB
Recommend Dilution	Western blot. Flow cytometry: 1/500 - 1/2000, use 10 µl of the suggested working dilution to label 1x10e6 cells in 100 µl (Membrane permeabilisation is required for this application, we recommend the use of Leukoperm). Immunohistochemistry on paraffin sections (requires antigen retrieval using heat treatment prior to staining, Sodium citrate buffer pH 6.0 is recommended). Does not work in ELISA.
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognises both native and recombinant IFN gamma.
Formulation	Phosphate buffered saline pH 7.4, 0.09% Sodium Azide State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 281237 Bovine UniProt ID P07353
Background	Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms	IFN-gamma, gamma IFN
Reference Data

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