Eph receptor B2 (EPHB2) Mouse Monoclonal Antibody [Clone ID: 48CT12.6.4]

Specifications

Product Data
Clone Name	48CT12.6.4
Applications	FC, IHC, WB
Recommend Dilution	Western blotting: 1/1,000. Immunohistochemistry: 1/200. Flow Cytometry: 1/10 - 1/50.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified His-tagged EPHB2 protein (Fragment between amino acids 124~425).
Specificity	This antibody is specific to EPHB2.
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	117493 Da.
Gene Name	EPH receptor B2
Database Link	Entrez Gene 2048 Human UniProt ID P29323
Background	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.
Synonyms	Ephrin type-B receptor 2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5, NY-REN-47
Reference Data

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