Phospholipase C gamma 1 (PLCG1) (970-990) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2D5]

Specifications

Product Data
Clone Name	2D5
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 of PLCgamma (Phospholipase C gamma).
Specificity	This monoclonal PLCG antibody (Clone 2D5) specifically recognizes Phospholipase C gamma at 150 kDa in Western blot applications.
Formulation	PBS State: Purified State: Lyophilized purified Ig fraction. Stabilizer: PEG and Sucrose Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 to -80°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	150 kDa
Gene Name	phospholipase C gamma 1
Database Link	Entrez Gene 18803 MouseEntrez Gene 25738 RatEntrez Gene 5335 Human UniProt ID P19174
Background	Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
Synonyms	Phospholipase C gamma, PLC-gamma
Note	Protocol: Positive Control Provided: Cell lysate from untreated SKOV-3 cells Description: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human) Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, T cell receptor signaling pathway, VEGF signaling pathway, Vibrio cholerae infection

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.