Cyclin B1 (CCNB1) Mouse Monoclonal Antibody [Clone ID: V92.1]

Specifications

Product Data
Clone Name	V92.1
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Immunohistochemistry on Frozen Sections: 1-2 μg/ml for 30 minutes at RT. Positive Control: Any human cell line in logarithmic growth phase, tonsil, or breast carcinoma.
Reactivity	Hamster, Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Hamster Cyclin B1 protein.
Specificity	This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. Cellular Localization: Cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	55-62 kDa
Gene Name	cyclin B1
Database Link	Entrez Gene 891 Human UniProt ID P14635
Background	Cyclins are evolutionarily conserved regulatory proteins that are essential for controlling the progression of the cell-cycle. Proliferating cells express both cyclin B1 and cyclin B2; expression is normally low in G0/G1, increases in S, and is maximal during the G2/M phase of cell division. Cyclin B1 can be found both on intracellular membranes and free in the cytoplasm, in contrast to cyclin B2, which is membrane-associated. Cyclin B1 binds to p34Cdc2, also known as Cdk1, a cyclin-dependent kinase, to form an inactive complex that accumulates in the cytoplasm. Dephosphorylation by Cdc25 results in activation of the complex and translocation to the nucleus. Cyclin B1 is rapidly degraded at the end of mitosis, and this degradation is essential for cells to exit from mitosis.
Synonyms	CCNB1, CCNB, Cyclin-B1
Reference Data

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