NNT1 (CLCF1) Rabbit Polyclonal Antibody
CAT#: AP01128BT-S
NNT1 (CLCF1) rabbit polyclonal antibody, Biotin
Size: 50 ug
Conjugation: Unconjugated
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CNY 3,910.00
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | ELISA: Direct: To detect hNNT-1/BCSF-3 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml is required. In conjunction with compatible secondary reagents, this antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3. Sandwich: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human NNT-1/BCSF-3 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98 %) recombinant human NNT-1/BCSF-3 |
Specificity | This antibody detects NNT-1/BCSF-3. |
Formulation | PBS, pH 7.2 Label: Biotin State: Sterile filtered lyophilized Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml. |
Purification | Affinity chromatography |
Conjugation | Biotin |
Storage Condition | Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing. |
Gene Name | cardiotrophin-like cytokine factor 1 |
Database Link | |
Background | NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. NNT1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. NNT1 may act as a neuroimmunoendocrine modulator of hypothalamus-pituitary-adrenal axis stress response. |
Synonyms | BSF3, CLC, NNT1, Novel neurotrophin-1 |
Reference Data | |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
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