Neurogranin (NRGN) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/8000 (Detection Limit). Western blot: Approx 16kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 7.6kDa according to NP_006167.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274(3):1294-300; PMID: 9880498).  Preliminary testing was unsuccessful on Mouse Brain for this particular batch. Recommended concentration: 0.1-0.3 µg/ml. Immunohistochemistry on Paraffin Sections: In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in the neuronal cell bodies. Recommended concentration: 5 µg/ml.
Reactivity	Bovine, Canine, Human, Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_006167.1.
Specificity	This antibody recognizes Neurogranin. Reported variants represent identical protein: NP_006167.1, NP_001119653.1
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	neurogranin
Database Link	Entrez Gene 4900 Human UniProt ID Q92686
Background	Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects.
Synonyms	Ng,Nrgn
Reference Data

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