BRCA1 / RNF53 (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1000.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 443-472 amino acids from the N-terminal region of Human BRCA1.
Specificity	This antibody recognizes Human BRCA1/RNF53 (N-term). Other species not tested.
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography, followed by peptide affinity purification
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	207721 Da
Gene Name	BRCA1, DNA repair associated
Database Link	Entrez Gene 672 Human UniProt ID P38398
Background	This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq].  Cellular Location: Nucleus. Chromosome (By similarity). Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex Isoform 5: Cytoplasm.  Tissue Location: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
Synonyms	BRCAI; BRCC1; BROVCA1; IRIS; PSCP; RNF53
Reference Data

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