Ptprc Mouse Monoclonal Antibody [Clone ID: OX-30]
CAT#: CL111PX
Ptprc mouse monoclonal antibody, clone OX-30, Purified
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CNY 6,567.00
货期*
5周
规格
Specifications
Product Data | |
Clone Name | OX-30 |
Applications | FC |
Recommend Dilution | Flow cytometry (see protocol). Immunohistochemistry with frozen sections. |
Reactivity | Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Lymph node glycoproteins and cells. Donor: BALB/c spleen Fusion Partner: NSO/U |
Specificity | This monoclonal antibody recognizes a monomorphic determinant of the rat leukocyte common antigen. |
Formulation | PBS and 0.02% NaN3 State: Purified State: Liquid purified Ig |
Concentration | lot specific |
Purification | Protein G Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | protein tyrosine phosphatase, receptor type, C |
Database Link | |
Background | The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170,000 kDa on thymocytes but molecular weight 170,000-220,000 kDa on other leukocytes. The leukocyte common antigen (L-CA) is a major glycoprotein of haematopoietic cells but is not found on other tissues or erythroid cells. It is present on greater than 95% of thymocytes, bone marrow cells and thoracic duct lymphocytes. This molecule carries much of the carbohydrate of thymocytes and shows interesting heterogeneity amongst T lymphocytes and B lymphocytes |
Synonyms | PTPRC, Leukocyte common antigen, L-CA, T200 |
Note | Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50µl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0.5-1.0 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1:700 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Wistar Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 0.5 µg/10e6 cells Isotypic Control: Mouse IgG2a Cell Source Percentage of cells stained above control: Thymus: 99.9% Spleen: 97.4% Lymph Node: 90.6% Results - Strain Distribution: Antibody Concentration Used: 0.5 µg/10e6 cells Strains Tested: Wistar, Buffalo, Brown Norway, Fischer 344 Positive: Wistar, Buffalo, Brown Norway, Fischer 344 Negative: none |
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