Estrogen Receptor 1 (ESR1) Rabbit Monoclonal Antibody [Clone ID: SP1]

Specifications

Product Data
Clone Name	SP1
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1:100 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Breast Carcinoma
Reactivity	Hamster, Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	Synthetic peptide derived from C-terminal of human estrogen receptor
Specificity	This antibody recognizes a protein of 67kDa, which is identified as estrogen receptor (ER). Cellular Localization: Nuclear.
Formulation	State: Supernatant State: Liquid tissue culture supernatant containing sodium azide as preservative
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	estrogen receptor 1
Database Link	Entrez Gene 2099 Human UniProt ID P03372
Background	ER content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Synonyms	ER alpha, Estradiol receptor, ESR1, ESR, NR3A1
Reference Data

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