LOC103694380 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: To detect rat TNF-alpha by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat TNF-alpha. Western Blot: To detect rat TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant rat TNF-α (rat TNF-alpha)
Specificity	Recognises Rat TNF alpha.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor-like
Database Link	Entrez Gene 103694380 Rat UniProt ID P16599
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Note	Centrifuge vial prior to opening!
Reference Data

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