EGFR Rat Monoclonal Antibody [Clone ID: ICR10]

Specifications

Product Data
Clone Name	ICR10
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Western blotting. Flow cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen sections. Recommended positive control: Breast carcinoma.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	Extracellular domain of human Epidermal Growth Factor Receptor (EGFR) from head and neck carcinoma.
Specificity	This antibody recognises the epidermal growth factor receptor (EGF-R), which is over expressed in a high proportion of breast cancer cells and in a range of other carcinomas. High level expression of EGFR is often associated with advanced disease and poor prognosis. This antibody binds to epitope B from EGFR (1, 2) and has an affinity of 6.7 x 10e-9 M.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Ion exchange chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data

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