Aqp2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid? residues 254-271 of rat AQP2.? Intracellular, C-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	aquaporin 2
Database Link	NP_037041 Entrez Gene 359 HumanEntrez Gene 11827 MouseEntrez Gene 25386 Rat UniProt ID P34080
Background	Aquaporin 2 (AQP-2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12. The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-2 together with AQP-1, AQP-4 and AQP-5 belongs to the first group. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores. AQP-2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP-2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine. Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressing receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP-2. This phosphorylation causes the translocation of AQP-2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.
Synonyms	AQP-2; AQP-CD; aquaporin-CD; MGC34501; WCH-CD
Reference Data

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