Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000
Host	Goat
Clonality	Polyclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity	Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum.  No reaction was observed against anti-papain or anti-Goat IgG F(c).
Formulation	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL - lot specific
Conjugation	Alkaline Phosphatase
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Background	Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
Synonyms	Goat Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugation, Goat Fab Anti-Biotin HRP Conjugated Antibody
Note	Fab Anti-Biotin Antibody Alkaline Phosphatase Conjugated has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.
Reference Data

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