Mouse IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody

Specifications

Product Data
Product Name	Mouse IgG (H+L chain), F(ab)2 Fragment Goat Polyclonal Antibody
Recommend Dilution	Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions ELISA: 1/20,000-1/100,000. Immunohistochemistry: 1/1,000-1/5,000. Western blot: 1/2,000-1/10,000.
Reactivity	Mouse
Host	Goat
Immunogen	Mouse IgG whole molecule.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer. Label: Biotin State: Lyophilized F(ab')2 fragments. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.