Human IGFBP6 ELISA Kit

Specifications

Product Data
Description	Human IGFBP6 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Human IGFBP6
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5pg/ml-4000pg/ml
Sample Type	Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of human IGFBP6
Sensitivity	<10pg/ml
Reactivity	Human
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- human IGFBP6 antibody 1 Lyophilized recombinant human IGFBP6 standard 10ng/tubex2 Biotinylated anti- human IGFBP6 antibody 130ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12ml ABC diluent buffer 12ml TMB color developing agent 10ml TMB stop solution 10ml Wash Buffer (25x) 20ml Plate Sealers 4x pieces
Background	Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
Gene Symbol	IGFBP6

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