Cldn11 (BC021659) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Claudin11, Claudin-11
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC021659 CCACGCGTCCGCGCTGCACGCTGTCGCAGCAGTGCTCGCAGCCGCTCTCTCCCCATCTCGAGTAGCCCGG AGCCAGCGGCTCGCGAGGGCCAAGAGGGCAAGCCTAGGGAAGGCTCTGTCCAGGACGACACAGGGGGCAC AATCCGTGTGAGTCGAGCTGCGTGGACGTCGCTGCGGCCACCATGGTAGCCACTTGCCTTCAGGTGGTGG GTTTCGTCACGAGCTTCGTGGGTTGGATTGGCATCATCGTCACAACGTCCACCAATGACTGGGTGGTGAC CTGCAGCTACACCATCCCCACCTGCCGAAAAATGGACGAACTGGGCTCCAAGGGCCTGTGGGCTGACTGC GTCATGGCCACTGGTCTCTACCACTGCAAACCCCTGGTGGACATCCTCATCCTTCCAGGCTACGTGCAGG CTTGTAGAGCCCTCATGATTGCTGCCTCCGTTCTGGGCCTGCCCGCCATCTTGCTGCTGTTGACAGTTCT CCCCTGCATCCGAATGGGCCACGAGCCTGGAGTGGCCAAGTACAGGCGAGCCCAGCTGGCTGGGGTGCTC CTTATTCTGCTGGCTCTCTGCGCCATTGTCGCCACCATCTGGTTTCCTGTATGTGCCCACCGCGAGATCA CCATCGTGAGCTTTGGCTACTCGCTGTACGCAGGTTGGATCGGTGCTGTGATGTGCCTGGTGGGTGGCTG TGTCATCGTCTGCTGCTCCGGGGATGCACAGTCATTTGGAGAAAACCGTTTCTATTACTCTTCTGGTTCC AGCTCGCCAACGCATGCCAAGAGTGCCCATGTCTAAGAGGGCTGCTCCACTGCCCGCCGAGGTGCTGTAA ATGCTGGGCCTGGGCCCTGGGTTTGCTCGCCACAGTGGGGAGAAGCCCACTTCCCTGCCAGGCACTAAAG CCAAAGTTCTAGAAAGTATCCTGCCCCGGCATTTTGAAGTCGTAACACCCCACCCACCCACCCACCACTT CTTGGCTGCCTTAAAAGAAAGCTCTAGCTCAGTTAATGCCCACATAGTTTTCTCCTGGAGTTGCGGGCTG TGGCTGTTTGCTCTTTCCTCGGGCATTCCATTGTTGTTGATTAAAAAAATATTTTGTTTCTCTCTTAAAT TCAAATGTCTTGGGAACATTGCTGACTTGGGTGTGGATTGGGAAAGAAATAAAAGATGCTTTTCAAAGGG TTACCAACGACAGTGGAAGCCTTATAGAGACAGCTCTCTTCTCCCTTTCGGCTTAGTTTCAAGGTCACTT ATATATAAGAGATAGAAATGGATAGATTGGGAACACGGGTGGGAGGGGAACTCAGAGCTTTCCCTCCACG GGAAGCTTCTCTTTTATAAGTTGAGGGGTTGGGTGTCTTTTTTTTTTTTTAGTTTGCGATTTTACATTTT TCTGTACGTACTTTTTCAAGATTGATCATTTTTATAACCACGGGTTTCCTGAAAATTCTCAATTCACCAA TATGAAGGAAATGAACCAAGCAGACGTTAATATGCAATAAATAATAGTACGAAGATTATAACTTTAACTG ACTGCCCACGGTTTCCAGGTTTGTATGCTATAGTTTTTAATCCTATGGTTGCATATGCTTCAAATTAACA CATTTAAAAATCTTTTCTCCCCTTCTATTTCTGTCTCCATTCTGTTAGAGACCATGAAGCAGTATTGTTT AACATAAGTTGTACTGTTAAGTTTGGCTTCATGGGTGTAAACACCAATGGTCTGTCAGTGTCTAAGACTC TGGATACTGCAAGCTCCGTCCGGTGCATTTGTTCAGGTAAAATCTGTGCAATAAAATAACAAACTGTCTC CAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC021659
Insert Size	624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021659, AAH21659
RefSeq Size	1848 bp
RefSeq ORF	624 bp
Locus ID	18417
Gene Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a major component of CNS (central nervous system) myelin and plays an important role in regulating proliferation and migration of oligodendrocytes. The basal cell tight junctions in stria vascularis are primarily composed of this protein, and the gene-null mice suffer severe deafness. This protein is also an obligatory protein for tight junction formation and barrier integrity in the testis and the gene deficiency results in loss of the Sertoli cell epithelial phenotype in the testis. [provided by RefSeq, Aug 2010]

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