Tfb1m (NM_146074) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI429207
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC032930 sequence for NM_146074 ATGGCTGCCTCGGGGAAGCTCGGCACTTTCCGCCTTCCCCCGTTGCCCACCATTCGGGAGATCATTAAGT TGTTCGGACTGCGAGCGGTGAAACAGCTTTCCCAGAATTTCCTCCTGGACTTGAGGCTGACAGACAAGAT TGTAAGGAAAGCTGGCAGTCTAGCAGATGTTTATGTTTATGAAGTGGGTCCTGGGCCCGGGGGAATCACA AGATCCATCCTGAATGCCAACGTTGCTGAGCTTCTGGTGGTTGAAAAGGACACTCGCTTTATCCCAGGGT TACAGATGCTCTCTGATGCAGCACCTGGAAAACTCCGAATTGTTCATGGAGATGTGCTGACATACAAGAT AGAAAAGGCTTTTCCAGGCAACATTAGAAGACAGTGGGAAGATGATCCTCCAAATGTACACATTATTGGA AATCTACCTTTTAGTGTTTCAACTCCACTGATTATCAAGTGGCTTGAAAATATTTCTCTTAAAGATGGCC CTTTCGTTTATGGCAGAACTAAGATGACGTTAACGTTTCAAAAGGAAGTGGCTGAGAGACTTGTAGCCAC TACAGGAAGCAAACAGCACAGTCGCCTTTCCATTATGGCCCAGTACCTCTGCAATGTTGAACACCTCTTT ACAATCCCAGGAAAAGCTTTTGTTCCCAAACCAAAAGTTGATGTAGGAGTGGTGCACCTTACGCCCTTGA TAGAGCCCAAGATCAAGCAGCCATTCAAGCTGGTAGAAAAAGTTGTCCAGAATGCGTTTCAGTTTCGAAG GAAATATTGCCACCGAGGGCTTGGAATGTTATTCCCTGAAGCACAACGCCTCGAAAGCACTGGAAGGCTG TTACAGTTGGCAGACATTGATCCAACTCTCCGGCCCACCCACCTCTCGCTCATGCATTTTAAGAGTTTGT GTGATGTATACAGAAAAATGTGTGATGAAGACCCACAACTCTTTACTTATAACTTCAGAGAGGAGCTCAA GCAAAAGAAAAGTAAAGGCCAAGAAAAAGATGGTGACCCAGAAAGCTGCGGGTTCTAATTGCTGCCTTAG GGCTCCCGCCTGCCAGGACACCCTGTGGACAGTGTGCATCCCCACAGATGAGTGTGCATCTCCCTACCTC TCTGTAAAGACAGATACCGATGACCTCGTGACTTTACTATCCAGCTTGGTAAGAAAAGAAATACCAAGTA GATACAGTGCAAGCTTTCCTTTAATGTATATACTGCATAGGTACGACATGTTTAGTAAAAATAGCATTTA TGACAACCTTCTAAAATGAAATATCTGTACGTTGTGCCTCTAAACCATGTAAAAATAAAAGTGCTTTAAA TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	NM_146074
Insert Size	1038 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC032930, AAH32930
RefSeq Size	1360 bp
RefSeq ORF	1038 bp
Locus ID	224481
UniProt ID	Q8JZM0
Gene Summary	S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity (By similarity).[UniProtKB/Swiss-Prot Function]

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